Table 1.
The information of nucleotide sequences of the primers used in this study, and the purpose of corresponding genes. Prophenoloxidase-activating enzyme (PPAE); prophenoloxidase (PPO).
| Gene | Primers | Purpose | Sequence (5′–3′) |
|---|---|---|---|
| serpin1 | serpin1-F | Expression | CGCGGATCC GATGCCAGTCCGCGCCTTCTC |
| serpin1-R | Expression | CCC AAGCTT TTGCGGAGGC CTTTGTGG | |
| qPCR-serpin1-F | Real-Time PCR | TACGCAGGCAAAGGAAAG | |
| qPCR-serpin1-R | Real-Time PCR | ATGGGTTTACGGTGCTC | |
| dsSerpin1-F | RNAi | TAATACGACTCACTATAGGATCAGCACAGCCAGGAAAC | |
| dsSerpin1-R | RNAi | TAATACGACTCACTATAGGCGGCATCGGAGAAGTATTG | |
| Actin | actin-F | Real-Time PCR | GTTACAAACTGGGACGACAT |
| actin-R | Real-Time PCR | AGAAAGCACAGCCTGAATAG | |
| PPAE | PPAE-F | Real-Time PCR | CACCAGCACAAATGAATGAC |
| PPAE-R | Real-Time PCR | CAACGACAATGAGGCACAG | |
| PPO | PPO-F | Real-Time PCR | AAAGACCGCAGAGGAGAA |
| PPO-R | Real-Time PCR | CCAACGATAGAACACAGGA | |
| defensin | defensin-F | Real-Time PCR | CCAGAAAGCGATGATGCCACTA |
| defensin-R | Real-Time PCR | CACCACAAATCAACGCCAAAGT |