Figure 1.

Inhibition of B16- BL6 cell proliferation treated with Uncaria tomentosa extracts: (A) B16-BL6 melanoma cells were treated with Uncaria tomentosa extracted with 70% ethanol or PBS at 4, 40, or 100 μg/mL on day one of treatment and relative cell viability determined every day over a period of 4 days using an MTT assay; (B) the relative viability of B16-BL6, NIH3T3, and C2C12 cells was determined for cells treated with Uncaria tomentosa extracts for 4 days using an MTT assay and the percent inhibition of cell viability was determined compared to media controls; (C) phase contrast micrographs of cell cultures treated with Uncaria extracts for 3 days showing changes in cell confluency as presented in the bar graph; and (D) fluorescence microscopic images of Ki-67-stained B16-BL6 cells and phase contrast images were taken on day 3 of treatment with Uncaria tomentosa extracts and plotted as the % of cells that were positive for KI-67 staining. Data were analyzed for three independent experiments and differences from control were considered significant (*) when p ≤ 0.05 by a Tukey post hoc test.