Figure 5.

PrP^C-Apt DOA inhibit mitochondrial function of CRC cells to a greater degree, as compared to free Dox. (A) Mitochondrial oxygen consumption rates (OCRs) of SNU-C5 cells treated with free Dox or PrP^C-Apt DOA were measured over time (min). The cells were treated with oligomycin (1 μM, 20 min), FCCP (0.75 μM, 40 min), and antimycin A (1 μM, 60 min) to obtain bioenergetic parameters. (B) Basal respiration was measured by subtracting non-mitochondrial OCR (which is the remaining OCR post antimycin A treatment) from the initial OCR level. (C) Maximal respiration was measured upon addition of FCCP. (D) Proton leak was calculated by subtracting the OCR post antimycin A treatment from the OCR post oligomycin A treatment. (E) Adenosine triphosphate (ATP) production was determined by subtracting non-mitochondrial OCR from the OCR value of basal respiration. The histogram shows representative data from one replicate experiment (n = 5). The values represent mean ± SEM. ** p < 0.01 vs. control; # p < 0.05, ## p < 0.01 vs. free Dox.