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Somatic embryogenesis (SE) initiation in sugi: (A) extrusion of embryogenic cells (ECs) about 2 weeks after culture initiation on media with plant growth regulators (PGRs), (B) EC proliferation on a medium with PGRs approximately 4 weeks after extrusion, and (C) germination and SE initiation on a medium with no PGRs approximately 4 weeks after culture initiation. Bars: 5 mm.
