Table 1.
Somatic embryogenesis (SE) initiation frequency from open-pollinated-derived seeds of the male-fertile family of sugi. The data represent the explants with SE initiation response and the total number of explants tested, and the number in the parentheses represent the initiation frequency (%) for each culture medium with or without plant growth regulators (PGRs) at different seed collection times.
| Mother Tree | Culture Medium | SE initiation Frequency (%) by Seed Collection Time | ||||
|---|---|---|---|---|---|---|
| Mid-June | Late-June | Early-July | Mid-July | Total | ||
| “Yanase 104” | PGRs (−) | 1/75 (1.33) |
3/60 (5.00) |
6/60 (10.00) |
9/75 (12.00) |
19/270 (7.04) ns |
| “Yanase 104” | PGRs (+) | 3/75 (4.00) |
7/90 (7.78) |
10/90 (11.11) |
11/75 (14.67) |
31/330 (9.39) ns |
| Total | 4/150 (2.67) * |
10/150 (6.67) ns |
16/150 (10.67) ns |
20/150 (13.33) ☥ |
50/600 (8.33) |
|
PGRs (−): explants cultured on medium without plant growth regulators; PGRs (+): explants cultured on medium with plant growth regulators; ns: no significant differentiation at p > 0.05 by post hoc analysis of Pearson’s Chi-squared test; * significantly different at p < 0.05 by post hoc analysis of Pearson’s Chi-squared test; ☥ significantly different at p < 0.1 by post hoc analysis of Pearson’s Chi-squared test.