Table 3.
Somatic embryogenesis (SE) initiation frequency of sugi seed family carrying the male sterility gene MS1. The data represent the explants with SE initiation response and the total number of explants tested, and the number in the parentheses represent the initiation frequency (%) for each of four seed families at different seed collection times.
| Seed Family $ | Collection Year | SE Initiation Frequency (%) by Seed Collection Time | |||
|---|---|---|---|---|---|
| Early-July | Mid-July | Late-July | Total | ||
| ♀ “Shindai 3” ♂ “Suzu 2” |
2016 | 108/300 (36.00) |
59/420 (14.05) |
45/516 (8.72) |
212/1236 (17.15) *** |
| ♀ “Shindai 3” ♂ “Suzu 2” |
2017 | 54/156 (34.62) |
191/383 (49.87) |
39/168 (23.21) |
284/707 (40.17) *** |
| ♀ “Fukushima-funen 1” ♂ “S3-37(1)” |
2017 | 2/324 (0.62) |
62/516 (12.02) |
17/216 (7.87) |
81/1056 (7.67) *** |
| ♀ “Fukushima-funen 1” ♂ “Oi 7” |
2017 | 11/156 (7.05) |
259/636 (40.72) |
85/144 (59.03) |
355/936 (37.93) *** |
| ♀ “Fukushima-funen 1” ♂ “S3-118(2)” |
2017 | 29/120 (24.17) |
191/429 (44.52) |
196/468 (41.88) |
416/1017 (40.90) *** |
| Total | 204/1056 (19.32) *** |
762/2384 (32.00) *** |
382/1512 (25.26) ns |
1348/4952 (27.22) |
|
$: All female and male parents had ms1/ms1 and Ms1/ms1 genotype, respectively; ns: no significant differentiation at p > 0.05 by post hoc analysis of Pearson’s Chi-squared test; *** significantly different at p < 0.001 by post hoc analysis of Pearson’s Chi-squared test.