Table 5.
Somatic embryogenesis (SE) initiation frequency from polycross-pollinated-derived seeds of sugi. The data represent the explants with SE initiation response and the total number of explants tested, and the number in the parentheses represent the initiation frequency (%) for each polycross family at different seed collection times.
| Polycross Family 1 | SE Initiation Frequency (%) by Seed Collection Time | |||
|---|---|---|---|---|
| Early-July | Mid-July | Late-July | Total | |
| “S 11” × “3 Mix” | 157/404 (38.86) |
583/819 (71.18) |
471/841 (56.00) |
1211/2064 (58.67) *** |
| “S 11” × “10 Mix” | 215/514 (41.83) |
451/726 (62.12) |
345/773 (44.63) |
1011/2013 (50.22) *** |
| Total | 372/918 (40.52) *** |
1034/1545 (66.93) *** |
816/1614 (50.56) *** |
2222/4077 (54.50) |
1: Mother tree and pollen parents used for polycross seed families are shown in Supplementary Table S3; *** significantly different at p < 0.001 by post hoc analysis of Pearson’s Chi-squared test.