Table 6.
Somatic embryogenesis (SE) initiation frequency of megagametophyte explants derived from seventeen male-fertile, eight male-sterile, and five polycross seed families of sugi. The data represent the explants with SE initiation response and the total number of explants tested, and the numbers in the parentheses represent the initiation frequency (%) for each seed family.
| Seed Family 1 | SE Initiation Frequency (%) of Seed Explants Derived From |
||
|---|---|---|---|
| Male-Fertile Family |
Male-Sterile Family |
Polycross Family |
|
| Chiyoda 327 (OP) | 12/100 (12.00) | ||
| Kuji 6 (OP) | 9/60 (15.00) | ||
| Kuji 9 (OP) | 1/74 (1.35) | ||
| Kuji 14 (OP) | 545/1224 (44.53) | ||
| Kuji 17 (OP) | 17/264 (6.44) | ||
| Kuji 39 (OP) | 5/94 (5.32) | ||
| Naka 3 (OP) | 41/192 (21.35) | ||
| Naka 5 (OP) | 10/122 (8.20) | ||
| Naka 6 (OP) | 18/78 (23.08) | ||
| Nihari 2 (OP) | 71/426 (16.67) | ||
| Taga 2 (OP) | 191/502 (38.05) | ||
| Taga 4 (OP) | 37/192 (19.27) | ||
| Taga 10 (OP) | 2/24 (8.33) | ||
| Taga 14 (OP) | 195/580 (33.62) | ||
| Tsukuba 2 (OP) | 27/192 (14.06) | ||
| Yamazaki 5 (OP) | 23/156 (14.74) | ||
| Yanase 104 (OP) | 105/750 (14.00) | ||
| “Shindai 3” × “Suzu 2” | 496/1943 (25.53) | ||
| “Fukushima-funen 1” × “S3-37(1)” | 81/1056 (7.67) | ||
| “Fukushima-funen 1” × “Oi 7” | 355/936 (37.93) | ||
| “Fukushima-funen 1” × “S3-118(2)” | 416/1017 (40.90) | ||
| “S1S1-35” × “Gosenshi 1” | 10/74 (13.51) | ||
| “S1S1-23(1)” × “Gosenshi 1” | 1/36 (2.78) | ||
| “S1S1-10(1)” × “Gosenshi 1” | 54/186 (29.03) | ||
| “S1S1-51(1)” × “Gosenshi 1” | 14/347 (4.03) | ||
| “S 1” × “3 Mix” | 32/286 (11.19) | ||
| “S 1” × “9 Mix” | 28/268 (10.45) | ||
| “S 11” × “3 Mix” | 1301/2280 (57.06) | ||
| “S 11” × “10 Mix” | 1031/2068 (49.85) | ||
| “G 1” × “10 Mix” | 40/478 (8.37%) | ||
| Total | 1309/5030 (26.02) *** | 1427/5595 (25.50) *** | 2432/5380 (45.20) *** |
1: Mother trees and pollen parents used for polycross seed families are shown in Supplementary Table S3; OP: open-pollinated; *** significantly different at p < 0.001 by post hoc analysis of Pearson’s Chi-squared test.