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Expressions of myogenic related genes in IgLON5wt and IgLON5kd C2C12 cells. IgLON5wt and IgLON5kd cells were cultured in differentiation medium for 0, 2, 4, and 6 days. mRNA and protein expressions of (A) COL1a1, FMOD, DPT, and THBS1, (B) ITM2a and CDH15, and (C) WASP were determined by real-time RT-PCR, Western blot, and immunocytochemistry. Controls are baseline values. Wt indicates cells transfected with scrambled vector. Means ± SD (n ≥ 3). ** p < 0.01. (Scale bar, 32 µm).
