Figure 4.

EV from TSG-primed WJ-MSCs ameliorates DSS-induced colitis in mice. CTL-EV and TSG-EV (200 μg in 200 μL PBS) or concentrated-CM derived from GW4869 treated MSCs (vehicle control, 200 μL) were injected intraperitoneally on day 1, 3 and 5 after mice were administered 3% DSS. (A) A schematic image of DSS-induced colitis experiment. (B). Mice were monitored for changes in (B) body weight and (C) DAI score (D) survival rate. (E,F) Mice were sacrificed at day 10 and colon lengths were measured. (G) H&E staining of colon sections and (H) histological scores are shown. Scale bar, 100 μm. (I) Neutrophil infiltration was determined by measuring colonic MPO activity on day 10. n = 3 mice per group. Results were shown as mean ± S.D. (* p < 0.05, ** p < 0.01, *** p < 0.001)