Skip to main content
. 2021 Feb 18;10(2):434. doi: 10.3390/cells10020434

Figure 3.

Figure 3

Benzbromarone, zafirlukast, and cilnidipine inhibit the release of mature IL-1β in rat microglial or THP-1 cells. (A) Western blot analysis of precursor and mature IL-1β in lysates (production) and supernatants (release) stimulated by LPS (500 ng/μL; 3 h) and ATP (2 mM; 30 min) in rat microglial cells. Each compound (10 μM; benzbromarone (BBR), zafirlukast (ZFK), cilnidipine (CNP), or A-438079 (A43; a selective P2X7R antagonist)) was treated 30 min before ATP stimulation. (B) The inhibitory effect of each compound compared to LPS (500 ng/μL; 3 h) and ATP (2 mM; 30 min)-induced release of mature IL-1β from rat microglial cells by ELISA analysis (n = 5–17, ### p < 0.001 vs. LPS, ** p < 0.01 vs. control). (C) The inhibitory effect of each compound compared to LPS (500 ng/μL; 3 h) and BzATP (300 μM; 30 min)-induced release of mature IL-1β from PMA-differented THP-1 cells by ELISA analysis (n = 4–10, ### p < 0.001 vs. LPS, * p < 0.05, ** p < 0.01 vs. control). Data are represented as mean ± SEM.