Figure 1.

TET3 knockdown reduced 5hmC after transient MCAO. Transient MCAO in adult mice significantly increased 5hmC between 5 min and three days of reperfusion compared to sham (n = 3/group) *p < 0.05 by Kruskal–Wallis (Dunn’s post hoc) test (a). Visualization of Cy3-tagged siRNA in rostral to caudal brain sections (1.54 to –2.18 from bregma) 24 h following intracerebral injection in adult mice (b). Intracerebral injection of TET3 siRNA significantly reduced TET3 protein levels in the cortex two days following treatment compared with negative control siRNA (Neg siRNA)-treated cohort (n = 6/group). *p < 0.05 by Mann–Whitney U test (c). Levels of TET3 mRNA from the peri-infarct cortex were increased significantly in mice subjected to transient MCAO at 6 h of reperfusion compared to sham. TET3 siRNA treatment significantly reduced TET3 mRNA levels compared with Neg siRNA–treated cohort (n = 3/group; (d)). Transient MCAO significantly increased 5hmC in the peri-infarct cortex at 6 h of reperfusion which was abrogated with TET3 siRNA treatment (n = 5–6/group; (e)). *p < 0.05 compared with sham and #p < 0.05 compared with Neg siRNA by Kruskal–Wallis (Dunn’s post hoc) test ((d) and (e)). Values in the histograms are mean ± SD.

TET: ten-eleven translocase.