Figure 4.

TET3 knockdown reduced post-ischemic 5hmC at the promoters of neuroprotective genes. DhMRs significantly altered in the promoters between the TET3 siRNA and Neg siRNA groups at 6 h of reperfusion following transient MCAO (n = 3/group) for total promoter regions (a) and mRNA promoters (b) in the peri-infarct cortex. Gene ontological analysis of biological pathways of reduced DhMRs at gene promoters following TET3 knockdown compared to negative siRNA treatment at 6 h of reperfusion following transient MCAO ((c) and (d)). Top 10 biological pathways ranked by enrichment score (c) and by gene ratio (genes/pathway) (d). Statistically significant DhMRs were identified by differential analysis with a cut-off of log₂ FC = 1.0 and p < 0.05.

DhMR: differentially hydroxymethylated region.