Figure 6.

TET3 knockdown decreased gene expression of neuroprotective genes in adult male and female mice. Expression of selected neuroprotective genes displaying downregulated DhMRs with TET3 inhibition at 24 h of reperfusion was assessed by real-time PCR analysis in the peri-infarct cortex. Expression of genes with Log₂ fold change value of 10 or above (a) and (b)). Expression of genes with Log₂ fold change values of less than 10 (c) and (d)). Values in the histograms are mean ± SD (n = 3/group). *p < 0.05 compared to negative siRNA by Mann–Whitney U test.

Nedd4l: neural precursor cell expressed developmentally downregulated gene 4-like; Hspa12: heat shock 70 kDa protein 12; Sod2: superoxide dismutase 2; Prkce: protein kinase C epsilon; Aox3: aldehyde oxidase 3; Aldh: aldehyde dehydrogenase; Angptl2: angiopoietin like 2; Npr2: natriuretic peptide receptor 2; Smad: mothers against decapentaplegic; Fgf: fibroblast growth factor; Nrxn: neurexin; Neg siRNA: negative control siRNA; TET: ten-eleven translocase.