Figure 2.

Effect of curcumin on cell cycle distribution on CD166 + EpCAM + CSCs subpopulation and CD166-EpCAM- non-CSCs subpopulation of A549 and H2170 cell lines in both rescue (synergistic) and preventive (sensitisation) treatment groups. In rescue (synergistic) treatment group, the cells were treated with single and combined treatment on day 7 after the formation of colonies or spheroids. In the preventive (sensitisation) treatment groups the cells were treated with single and combined treatment, 48 h post-treatment. Cell cycle analysis was performed by flow cytometry. Each bar represents the average mean ± SD of triplicate samples. Statistical significance was measured with the two-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with untreated cells. # p < 0.05, ## p < 0.01, ### p < 0.001 compared with curcumin alone.