Table 4.
Effects of subretinal Aβ on the global thickness/structure of individual retinal layers as well as total retinal thickness assessed by optical coherence tomography. Measurements were carried out in mice subretinally injected with either Aβ1-42 (n = 7) or vehicle (n = 6) after 1 or 2 weeks. Data shown as an average percentage change from baseline +/- the standard error of the mean (SEM) to account for intra-animal variability. Statistical analysis using the Mann–Whitney U test revealed no change in the RNFL (p = 0.1375, p = 0.1807), GCLIPL (p = 0.3660, p = 0.5338), INL (p = 0.4452, p = 0.1014), OPL (p = 0.2343, p = 0.3660), ONL (p = 0.6282, p = 0.9452), IS (p = 0.3660, p = 0.7308), OS (p = 0.4452, p = 0.5338), ETPRS (p = 0.9452, p = 0.8357), RPE (p = 0.3660, p = 0.7308) or total RT (p = 0.7308, 0.9452) at week one and two, respectively. RNFL: Retinal Nerve Fiber Layer; GCIPL: Ganglion Cell Inner Plexiform Layer; INL: Inner Nuclear Layer; OPL: Outer Plexiform Layer; ONL: Outer Nuclear Layer IS: Inner Segments; OS: Outer Segments, ETPRS: End Tips of Photoreceptors; RPE: Retinal Pigment Epithelium; RT: total Retinal Thickness (the sum of all the layers measured).
| Retinal Layer | Change in Layer Thickness (%) | |||
|---|---|---|---|---|
| Week 1 | Week 2 | |||
| Vehicle | Aβ | Vehicle | Aβ | |
| RNFL | −9.2 ± 5.9 | −22.9 ± 5.3 | −21.9 ± 4.4 | −31.8 ± 6.8 |
| GCL/IPL | −7.0 ± 3 | −4.4 ± 2.6 | −10.7 ± 3.2 | −7.2 ± 2.5 |
| INL | 7.1 ± 4.5 | 14.6 ± 4.8 | 2.5 ± 4.1 | −4.7 ± 2.9 |
| OPL | 5.4 ± 9.4 | −12.9 ± 10.4 | −18.9 ± 10.9 | 13.0 ± 31.6 |
| ONL | −2.1 ± 6.0 | −0.06 ± 7.9 | 0.007 ± 8.0 | −2.0 ± 6.2 |
| IS | 23.5 ± 14.7 | 3.65 ± 9.0 | −4.0 ± 10.4 | 8.6 ± 10.6 |
| OS | −2.8 ± 8.3 | −9.6 ± 6.3 | 1.3 ± 9.5 | −10.7 ± 8.0 |
| ETPRS | −4.1 ± 13.1 | −7.1 ± 7.6 | −4.5 ± 13.8 | −7.3 ± 7.7 |
| RPE | 10.3 ± 21.5 | −14.2 ± 18.8 | −5.5 ± 14.2 | −22.4 ± 8.4 |
| RT | −2.2 ± 2.4 | −3.6 ± 2.1 | −2.9 ± 1.6 | −3.1 ± 2.1 |