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. 2021 Feb 13;10(2):199. doi: 10.3390/pathogens10020199

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glycotyping of serotype 1/2a L. monocytogenes strains of ST321 and ST391 strains investigated in this study. N-acetylglucosamine (GlcNAc) and rhamnose (Rhamn) were detected using GFP-labeled CBDP35 and A006_gp17 [32]. The green signal indicates the presence of the respective WTA substituent. Sequence type (ST) designations are in parentheses underneath the strain designation. R (red font) and S (green font) indicates phage resistance and susceptibility, respectively, to the four wide-host-range phages employed in the study, i.e., A511, P100, 20422-1 and 805405-1. The glycotyping assay was done as described in Materials and Methods.