Table 1.
Gene | Direction | Primer Sequence | Reference |
---|---|---|---|
Gapdh | Forward | 5′-GGCACAGTCAAGGCTGAGAATG-3′ | NM_017008.3 * |
Reverse | 5′-ATGGTGGTGAAGACGCCAGTA-3′ | ||
Col1a1 | Forward | 5′-GACATGTTCAGCTTTGTGGACCC-3′ | NM_053304 * |
Reverse | 5′-AGGGACCCTTAGGCCATTGTGTA-3′ | ||
Col1a3 | Forward | 5′-TTTGGCACAGCAGTCCAATGTA-3′ | NM_032085 * |
Reverse | 5′-GACAGATCCCGAGTCGCAGA-3′ | ||
Tgf-β1 | Forward | 5′-CACCGGAGAGCCCTGGATA-3′ | NM_021578 * |
Reverse | 5′-TCCAACCCAGGTCCTTCCTA-3′ | ||
α-Sma | Forward | 5′-ATCCTGACCCTGAAGTATCCGATA-3′ | NM_031004 * |
Reverse | 5′-CCACGCGAAGCTCGTTATAGA-3′ | ||
Smad3 | Forward | 5′-CGCATGAGCTTCGTCAAAGG-3′ | NM_013095.3 * |
Reverse | 5′-CCGATCCCTTTACTCCCAGTG-3′ | ||
Il-1β | Forward | 5′-CACCTCTCAAGCAGAGCACAG-3′ | M98820 * |
Reverse | 5′-GGGTTCCATGGTGAAGTCAAC-3′ | ||
Il-6 | Forward | 5′-TCCTACCCCAACTTCCAATGCTC-3′ | E02522 * |
Reverse | 5′-TTGGATGGTCTTGGTCCTTAGCC-3′ |
* GenBank accession numbers