Table 1.
The SNP and thermal conditions for analysis.
| Gene, SNP | Primer Sequence F-Forward, R-Reverse |
Thermal Conditions | Fragments, bp |
|---|---|---|---|
| MDM2 rs2279744 | F 5′-CGCGGGAGTTCAGGGTAAAG-3′ R 5′-CTGAGTCAACCTGCCCACTG-3′ |
5 min of denaturation at 95 °C, then 35 cycles of 95 °C for 30 s, 63 °C for 30 s, and 72 °C for 30 s. The final cycle had a 7 min extension at 72 °C | 157 |
| MDM2 rs937283 | F 5′-TGACCGAGATCCTGCTGCTTTC-3′ R 5′-TGAGTCAACCTGCCCACTGAAC-3′ |
5 min of denaturation at 95 °C, then 35 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s. The final cycle had a 7 min extension at 72 °C | 617 |
| MDM2 rs937282 | F 5′-GGTAACAGCGACACGGAGAT -3′ R 5′-CTCCGGGATGATGGAGTG -3′ |
5 min of denaturation at 95 °C, then 35 cycles of 95 °C for 30 s, 57,5 °C for 30 s, and 72 °C for 30 s. The final cycle had a 7 min extension at 72 °C | 231 |
| MDM4 rs1380576 | F 5′-GAAGAGGTGACATTTAACCTGAGACTT-3′ R 5′-GTGGTCTATCCCCTCAGCACATTTCCA-3′ |
5 min of denaturation at 94 °C, then 35 cycles of 94 °C for 30 s, 56,6 °C for 45 s, and 72 °C for 30 s. The final cycle had a 10 min extension at 72 °C | 195 |
| MDM4 rs4245739 | F 5′-AAGACTAAAGAAGGCTGGGG-3′ R 5′-TTCAAATAATGTGGTAAGTGACC-3′ |
3 min of denaturation at 94 °C, then 35 cycles of 94 °C for 30 s, 51 °C for 30 s, and 72 °C for 30 s. The final cycle had a 10 min extension at 72 °C | 134 |
Nucleotide base in underlined letter is mismatched base added in the primer sequence to create restriction enzyme site.