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. 2021 Feb 19;10(4):866. doi: 10.3390/jcm10040866

Table 2.

The SNP and restriction conditions for analysis.

Gene and SNP Restriction Endonuclease Restriction Conditions Fragments, bp
MDM2 rs2279744 MspA1I 37 °C for 1 h T allele 157;
G allele 109 + 48
MDM2 rs937283 MboI 37 °C for 1–16 h A allele 437 + 87 + 86 + 7;
G allele 524 + 86 + 7
MDM2 rs937282 Hpy188I 37 °C for 1 h C allele 231;
G allele 182 + 49
MDM4 rs1380576 BseNI (BsrI) 65 °C for 1–16 h G allele 195;
C allele 172 + 23
MDM4 rs4245739 MspI (HpaII) 37 °C for 1–16 h C allele 110 + 24;
A allele 134