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. 2021 Feb 18;22(4):2032. doi: 10.3390/ijms22042032

Figure 2.

Figure 2

Acetylated histone H3 was increased, but HDAC activity and expression levels of HDAC4, HDAC5, HDAC10, HDAC11, SIRT6, and SIRT7 were decreased in intrinsically aged human skin in vivo. The buttock skin of young and elderly subjects was obtained by punch biopsy. Immunofluorescence analysis of AcH3 and DAPI staining of paraffin-embedded sections (n = 6). Original magnification: ×200 (a). Western blot analysis of AcH3 and β-actin. The bands are representative of the results obtained for 10 young and 10 elderly subjects (n = 10) (b). Total HDAC activity was measured using an HDAC assay kit (n = 10) (c). Expression levels of HDAC and SIRT mRNAs were compared in young (n = 10) and aged (n = 10) subjects by qPCR (d,e). Data were normalized to one of the young subjects. Data are presented as means ± SEM. Statistical significances were determined using a two-tailed Student’s t-test. * p < 0.05, ** p < 0.01 vs. YB. YB: young buttock skin; OB: old buttock skin.