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. 2021 Feb 18;22(4):2032. doi: 10.3390/ijms22042032

Figure 3.

Figure 3

Acetylated histone H3, HDAC activity, and expression of HDACs and SIRTs did not show significant changes in photoaged human skin in vivo. Buttock and forearm skin of the same elderly subjects were obtained by punch biopsy. Immunofluorescence analysis of AcH3 and DAPI (n = 6). Original magnification: ×200 (a). Western blot analysis of AcH3 and β-actin protein (n = 9). The bands are representative of the results obtained in nine independent experiments (b). Total HDAC activity was measured using an HDAC assay kit in control or UV-irradiated human skin tissues (n = 10) (c). Expression levels of HDAC and SIRT mRNAs were compared in buttock and forearm skin of the same aged subjects by qPCR (n = 10) (d,e). Data were normalized to each individual’s buttock skin. The bar graph shows the means ± SEM. Statistical significances were determined using a two-tailed paired t-test. OB: Old buttock skin; OF: Old forearm skin.