Table 3.
Primers for sgRNA detection by real-time PCR.
| Target Viral Gene | Primer | Primer Sequence | Reference Regions (5′-3′ Nucleotides) |
|---|---|---|---|
|
Sg-N [7,14] (TaqMan) |
Forward | CAACCAACTTTCGATCTCTTGTA | 18–40 |
| Reverse | TCTGCTCCCTTCTGCGTAGA | 28771–28790 | |
| Probe | 5′FAM-ACTTCCTCAAGGAACAACATTGCCA-BBQ-3′ | 28738–28763 | |
| SYBR-Green | Forward | CAAACCAACCAACTTTCGATCTCTTGTA | 12–40 |
| Reverse | TCTGGTTACTGCCAGTTGAATC | 29669–29693 | |
| Sg-E [12] (TaqMan) | Forward [1] | CGATCTCTTGTAGATCTGTTCTC | 29–51 |
| SYBR-Green | Reverse [1] | ATATTGCAGCAGTACGCACACA | 26346–26367 |
| Probe [1] | 5′FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ-3′ | 26318–26343 | |
| Forward | CAAACCAACCAACTTTCGATCTCTTGTA | 12–40 | |
| Reverse | AGAAGTACGCTATTAACTATT | 26266–26287 | |
| N_gene [7] (TaqMan) | Forward | CACATTGGCACCCGCAATC | 28692–28800 |
| Reverse | GAGGAACGAGAAGAGGCTTG | 26346–26367 | |
| Probe | 5′FAM-ACTTCCTCAAGGAACAACATTGCCA-BBQ-3′ | 28738–28763 | |
| SYBR-Green | Forward | GACCCCAAAATCAGCGAAAT | 28273–28293 |
| Reverse | TCTGGTTACTGCCAGTTGAATCTG | 28321–28345 |