Table 5.
Comparisons of the performances of each kit, referring to the different targets detected and reported as the threshold cycle (Ct) in Covid19-positive swabs.
| Sample ID |
SEEGENE (Kit-A) |
ANATOLIA (Kit-B) |
AB-ANALITICA * (Kit-C) |
IONEBIO (Kit-D) |
GENCURIX (Kit-E) |
Concordance (%) on the Target | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene N |
Gene E |
Gene RdRp |
Gene ORF1ab | Gene E |
Gene RdRp |
Gene E |
Gene N |
Gene RdRp | Gene E |
Gene E |
Gene RdRp | Gene N |
|
| (Ct) | (Ct) | (Ct) | (Ct) | (Ct) | (Ct) | (Ct) | (Ct) | (Ct) | (Ct) | % | % | % | |
| M3 | 33.18 | 30.26 | 31.68 | 24.02 | 24.05 | 23.2 | 22.8 | 3.96 | 23.87 | 24.35 | 100 | 100 | 100 |
| R | 22.46 | 18.81 | 19.78 | 22.1 | 22.2 | 21.6 | 21.5 | 4.18 | 22.19 | 23.18 | 100 | 100 | 100 |
| S | 23.42 | 20.92 | 22.44 | 26.03 | 26.06 | 27.1 | 26.5 | 4.04 | 25.15 | 25.7 | 100 | 100 | 100 |
| S3 | 19.75 | 16.03 | 17.43 | 19.2 | 19.03 | 19.2 | 18.9 | 3.91 | 19.82 | 20.24 | 100 | 100 | 100 |
| T1 | 23.58 | 21.67 | 22.67 | 25.3 | 25.1 | 25.1 | 24.8 | 9.29 | 25.44 | 26.0 | 100 | 100 | 100 |
| D | 33.18 | 30.26 | 31.68 | ND | ND | 33.6 | 33.1 | 10.78 | 39.84 | 34.63 | 75 | 100 | 100 |
| I3 | 33.19 | 29.6 | 32.25 | 34.05 | 34.03 | 34.1 | 33.6 | 26.04 | 35.4 | 35.5 | 100 | 100 | 50 |
| V2 | 34.75 | 31.19 | 37.02 | ND | ND | 34.6 | 34.1 | 25.0 | ND | 36.66 | 75 | 66 | 100 |
| E2 | 21.67 | 17.54 | 18.66 | 22.08 | 22.05 | 22.03 | 22.2 | 3.73 | 20.41 | 21.23 | 100 | 100 | 100 |
| C3 | 30.49 | 28.09 | 29.65 | 32.07 | 32. | 32.01 | 32.3 | 25.99 | 31.85 | 32.28 | 100 | 100 | 50 |
| G3 | 33.63 | 30.42 | 31.92 | ND | ND | 34.04 | 34.1 | 16.64 | ND | ND | 50 | 66 | 100 |
| L3 | 24.98 | 21.51 | 23.35 | 26.04 | 26 | 26.02 | 26.2 | 14.93 | 24.94 | 25.2 | 100 | 100 | 100 |
| 6 | 27.23 | 28.59 | 28.12 | 24.05 | 23.3 | 26.3 | 26.1 | 3.67 | 26.23 | 27.21 | 100 | 100 | 100 |
| 7 | 23.17 | 24.56 | 23.92 | 22.51 | 22.07 | 22.9 | 22.8 | 32.66 | 24.52 | 25.4 | 100 | 100 | 50 |
| 12 | 30.13 | 32.04 | 30.23 | 25.41 | 25.11 | 30.7 | 30.3 | 3.23 | 29.17 | 30.34 | 100 | 100 | 100 |
| 24 | 35.18 | 38.54 | 35.27 | ND | ND | 35.5 | 35.5 | 2.88 | ND | 37.23 | 75 | 66 | 100 |
| 145 | 37.34 | 38.18 | 36.51 | ND | ND | 34.7 | 34.1 | 3.22 | ND | ND | 50 | 66 | 100 |
| 146 | 29.04 | 31.11 | 29.16 | 24.48 | 24.5 | 28.2 | 27.02 | 3.03 | 28.72 | 29.33 | 100 | 100 | 100 |
| 149 | 35 | 36.81 | 34.25 | ND | ND | 34.7 | 33.9 | ND | ND | ND | 50 | 66 | 50 |
Kits compared and reported in Table 5: Ionebio; Allplex 2019-nCov Assay (SEEGENE); BOSPHORE Novel Coronavirus 2019-Ncov (ANATOLIA); RealQualityRQ-2019-NCOV (AB-ANALITICA, * = the reference method); GenePro COVID-19 GENCURIX; ND = not detected. The data marked ‘ND’ were treated as missing values of detection by the kit, and excluded/ignored from the correlation reported in Table 6 and Table 7. In detail, SPSS uses pairwise deletion of missing values: each correlation uses all of the cases having valid values for all of the of the variables.