Skip to main content
. 2021 Feb 18;10(2):431. doi: 10.3390/cells10020431

Figure 2.

Figure 2

KRASmut MIA PaCa-2 cells are more dependent on the hexosamine biosynthetic pathway (HBP) and glycosylation for their survival than KRASwt BxPC-3. (A) Protein O-GlcNAc and OGT expression were detected in total cell extracts from MIA PaCa-2 and BxPC-3 cells at 24, 48, and 72 h of culture. The expression of EIF2α was used for signal normalization. On the left, a representative image is shown, while the band intensity quantification of at least three independent blots is displayed in the histograms on the right. (B) MIA PaCa-2 and BxPC-3 cells were cultured in presence of different concentrations of 2-DG −/+ 1 mM mannose (MAN) and counted after 72 h using the Trypan Blue stain. (C) Representative microscopy images of MIA PaCa-2 and BxPC-3 cells upon 72 h-treatment with 2.5 mM 2-DG −/+ 1 mM MAN. All data represent the mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 (Student’s t-test), −MAN vs. +MAN in panel (B).