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. 2021 Feb 18;10(2):431. doi: 10.3390/cells10020431

Figure 4.

Figure 4

KRASmut MIA PaCa-2 and PANC-1 cells are more sensitive to the HBP inhibition mediated by FR054 than KRASwt BxPC-3. (A) Cell viability of BxPC-3, MIA PaCa-2 and PANC-1 cells treated for 48 h with different concentrations of FR054 was detected by viable count using Trypan Blue stain. (B,C) Cell death of BxPC-3, MIA PaCa-2 and PANC-1 treated with 0.5 mM FR054 was evaluated by viable count (B) and by using flow cytometric analysis of Annexin V-FITC and propidium iodide staining (C). Representative profiles of the flow cytometric analysis are shown on the right of panel (C). All data represent the mean ± SEM of at least three independent experiments. * p < 0.05 and ** p < 0.01 (Student’s t-test in panel (A); one-way ANOVA in panels (B,C)), MIA PaCa-2 and Panc-1 vs. BxPC-3 (black asterisks) and untreated vs. treated (grey asterisks) where not specifically indicated.