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. 2021 Feb 18;10(2):431. doi: 10.3390/cells10020431

Figure 6.

Figure 6

Oncogenic KRAS expression in NIH3T3 cells induces sensitivity to HBP and RAS inhibition. (A) Protein O-GlcNAc levels as well as the expression of the HBP proteins were analyzed through Western blotting in normal (N), transformed (T) and reverted (R) NIH3T3 cells. As a loading control, Ponceau staining and actin expression were used, respectively. Representative images are shown, while the band intensity quantification of at least two independent blots is displayed in the histograms. (B–F) Cell number (C,E) and cell death (B,D,F) of the NIH3T3 cells were evaluated through Trypan Blue assay upon 72 h treatment with 2.5 mM 2-DG −/+ 1 mM mannose (B), with different concentrations of FR054 (C,D), and with 350 µM FR054 −/+ 50 µM BI-2852 (E,F). Data represent the mean ± SEM of at least two independent experiments. * p < 0.05, ** p < 0.01 (one-way ANOVA), untreated vs. treated and T vs. N or R (see colored asterisks), where not specifically indicated.