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. 2021 Feb 17;10(2):307. doi: 10.3390/antiox10020307

Figure 3.

Figure 3

SMEF suppresses RANKL-induced NF-κB and AP-1 activation after inducing with RANKL for indicated time. (A–D) RAW 264.7 cells were pretreated with SMEF (50 μg/mL) for 12 h and then exposed to RANKL for 0, 10, 30, and 60 min. Cytosolic fractions were analyzed for protein content of IκB-α degradation, whole-cell lysates were used to determine phosphorylation levels of NF-κB p65, and nuclear fractions were analyzed for NF-κB p65 and AP-1(c-Jun) translocation (assayed by Western blotting as described in the Materials and Methods section).