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. 2021 Feb 17;22(4):1968. doi: 10.3390/ijms22041968

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Quantitation of fatty acid species in lipid subclasses of LPA treated BV-2 cells. Serum starved BV-2 cells were incubated in the absence and presence of 1 µM LPA for the indicated times. (A) Quantitation of total FAs present in the indicated lipid species (PL, phospholipids; DG, diacylglycerols; FFA, free fatty acids; TAG, triacylglycerols; CE, cholesterylesters). Individual FA content in each lipid class: (B) Phospholipids, (C) diacylglycerols, (D) free fatty acids, (E) triacylglycerols, and (F) cholesterylesters. Data represent mean ± SEM of four independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control; one-way ANOVA with Bonferroni correction).