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. 2021 Feb 17;22(4):1968. doi: 10.3390/ijms22041968

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Amino acid analysis of LPA treated BV-2 cells. Serum-starved BV-2 cells were incubated in the absence (control) and presence of LPA (1 µM) for the indicated times. HPLC analysis of OPA derivatized amino acids (AAs) was performed and amino acid concentrations were determined by area comparison with external calibration curves and subsequent normalization to the cellular protein content. Bar graphs indicate mean ± SEM of 3 independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to controls; Student’s t-test). The amino acids are grouped according to their chemical properties in: (A) Aliphatic AAs, (B) OH- and S-containing AAs, (C) aromatic AAs, (D) basic AAs, (E) acidic AAs, and (F) non-proteogenic AAs.

Amino acid analysis of LPA treated BV-2 cells. Serum-starved BV-2 cells were incubated in the absence (control) and presence of LPA (1 µM) for the indicated times. HPLC analysis of OPA derivatized amino acids (AAs) was performed and amino acid concentrations were determined by area comparison with external calibration curves and subsequent normalization to the cellular protein content. Bar graphs indicate mean ± SEM of 3 independent experiments. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to controls; Student’s t-test). The amino acids are grouped according to their chemical properties in: (A) Aliphatic AAs, (B) OH- and S-containing AAs, (C) aromatic AAs, (D) basic AAs, (E) acidic AAs, and (F) non-proteogenic AAs.