Figure 8.

Key metabolic pathways alterations in LPA-activated BV-2 microglia. Graphical summary of findings obtained during this study. Stimulation of microglia with LPA reprograms metabolism towards aerobic glycolysis for generation of ATP via the Akt/mTOR/HIF1α axis. As a consequence, lactate secretion increases. The activated BV-2 cells switch mitochondrial metabolism to support FA synthesis. Increased AA uptake can further fuel this metabolic program and sustain proinflammatory cytokine synthesis. In response to ROS generation the antioxidant response is initiated and results in higher levels of intracellular GSH to prevent damage of endogenous macromolecules. Red = decreased, green = increased. (Intermediates not experimentally addressed during the present study are shown in grey).