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Schematic diagram of the experimental procedure. Cells were seeded and kept serum-free over-night. One mL serum free media was added to all wells and 20 µL LPA [0.05 mM] was added at indicated times for a final concentration of 1 µM. For 5 µM LPA treatment, 1 µL LPA [5 mM] was added to 1 mL media. At the end of the treatment, cells were collected for different downstream analyses.
