Antibody
|
Company
|
Cat no
|
Dilution
|
Phospho Akt (Ser 473) |
Cell Signaling Technology |
cs-4060 |
1:1000 |
Akt |
Cell Signaling Technology |
cs-9272 |
1:1000 |
Phospho Mtor (Ser 2448) |
Cell Signaling Technology |
cs5536 |
1:1000 |
Mtor |
Cell Signaling Technology |
cs-2972 |
1:1000 |
HIF1α |
Cell Signaling Technology |
cs-14179 |
1:1000 |
Phospho AMPK(Thr 172) |
Cell Signaling Technology |
cs-2535 |
1:500 |
AMPK |
Cell Signaling Technology |
cs-2532 |
1:1000 |
Phospho ACC(Ser 79) |
Cell Signaling Technology |
cs-3661 |
1:1000 |
ACC |
Cell Signaling Technology |
cs-3676 |
1:1000 |
Phospho SREBP (Ser 372) |
Cell Signaling Technology |
cs-9874 |
1:1000 |
SREBP1 |
Abcam |
ab44153 |
1:1000 |
Phospho NRF2 (Ser 40) |
Abcam |
ab76026 |
1:1000 |
GCLm |
Santa Cruz Biotechnology Inc |
sc166603 |
1:1000 |
β-actin |
Santa Cruz Biotechnology Inc |
sc47778 |
1:5000 |
Gene
|
Company
|
Cat no
|
Forward/Reverse Primers
|
Glut2 |
Qiagen |
QT00103573 |
|
Glut3 |
Qiagen |
QT00159691 |
|
Glut5 |
Qiagen |
QT00148267 |
|
HPRT |
Qiagen |
QT00166768 |
|
GLUT1 |
Invitrogen |
|
F: CCGTTCTCCGTCTCGCAG |
|
|
|
R: CTCCCACAGCCAACATGAGG |
GLUT4 |
Invitrogen |
|
F: ATTGTCGGCATGGGTTTCCA |
|
|
|
R: AGCAGGAGGACGGCAAATAG |
Hexokinase2 |
Invitrogen |
|
F: ATCGCCTGCTTATTCACGGAG |
|
|
|
R: TCTGAGAGACGCATGTGGTAG |