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. 2021 Feb 18;22(4):2040. doi: 10.3390/ijms22042040

Figure 3.

Figure 3

Seeds of ami1 rty mutants show a severe growth arrest and accumulation of IAM and IAA. (A) The photographs show close-ups of wild type (wt) (Col-0) and ami1 rty seeds grown on ½ Murashige & Skoog plates. The arrows mark the tip of the primary root two days after emergence. At this stage, the ami1/rty root stops growing. Scale bars = 1 mm. (B) The figure shows the comparison of wt (Col-0) and ami1 rty mutant seeds grown on ½ MS plates. The ami1/rty seeds abort germination, while wt (Col-0) seeds were able to germinate. (C) Analysis of IAM and IAA levels in wild-type Arabidopsis, iaaMox, and ami1 rty. Imbibed seeds from wild-type Arabidopsis (Col-0), iaaMox, and the ami1 rty double mutant were analyzed for their endogenous IAM (upper panel) and IAA levels (lower panel). The plots show mean values with their corresponding standard errors (SE), n = 5. To assess significant differences between the endogenous levels of each compound an analysis of variance (anova) of the scores followed by Tukey’s post-hoc test was performed. The different letters indicate significant differences, p ≤ 0.001.