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. 2021 Feb 17;22(4):1984. doi: 10.3390/ijms22041984
amyloid β peptide
AD Alzheimer’s disease
Anti-CaM*A488/Anti-Cav1*A488/Anti-HRas*A488/Anti-LTCC*A488/Anti-PrPc*A488 primary antibody of target protein (CaM, Cav1, HRas, LTCC or PrPc) stained with a secondary Alexa488 fluorescent antibody
Anti-HRas*Cy3 primary antibody of HRas stained with a secondary Cy3 fluorescent antibody
BSA bovine serum albumin
[Ca2+]i intracellular free calcium concentration
CaM calmodulin
CaMKII Ca2+/calmodulin-dependent protein kinase isoform II
CaMBDs calmodulin binding domains
CGN cerebellar granule neurons
DMEM Dulbecco’s Modified Eagle medium
FRET fluorescence resonance energy transfer
Fura2-AM Fura2 acetoxymethyl ester
LTCC L-type calcium channels
MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra¬zolium bromide
nNOS neuronal isoform of nitric oxide synthase
PAGE polyacrylamide gel electrophoresis
PBS phosphate-buffered saline
PBST PBS supplemented with 0.2% Triton X-100
PMCA plasma membrane calcium pump
PrPc cellular prion protein
PVDF polyvinylidene difluoride
ROI region of interest
SDS sodium dodecyl sulfate
TBS Tris-buffered saline
TBST TBS supplemented with 0.05% polyoxyethylene sorbitan monolaurate (Tween 20)
Tris tris-(hydroxymethyl) aminomethane
Triton X-100 4-(1,1,3,3-tetramethyl butyl)phenyl-poly-ethylene glycol