Figure 2. The structure of IFNα and the introduced N-glycosylation site.
A) The structure of human IFNα (PDB code 2hym). The two positions chosen for site-directed mutagenesis are highlighted. Mutagenesis at P4 is shown in blue and at Q158 in red. B) The engineered N-glycosylation sites. The modified amino acids in both the mutants are highlighted in red.