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Assessment of LAMP assay sensitivity for the detection of Botryosphaeria dothidea. 1000 × SYBR Green I color change-based LAMP product detection (A). Agarose gel electrophoresis-based LAMP product detection (B). DNA isolated from Botryosphaeria dothidea isolate was subjected to serial 10-fold dilution (10 ng–10−6 ng) and served as a template in LAMP assays. M: DNA marker 2 k, 1–7: 10 ng, 10−1 ng, 10−2 ng, 10−3 ng, 10−4 ng, 10−5 ng, 10−6 ng, 8: water.
