Fig. 4.

CAFs reduced the level of DNA damage in NPC cells after irradiation. a GSEA of GSE48501 cell microarray and tissue microarray were conducted and pathways related to DNA damage and repair were enriched. b and c Comet assay showed that CAFs reduced the level of DNA damage in irradiated tumor cells. d and e Interruption of the IL-8 signaling pathway increased the distribution of γ-H2AX foci post-irradiation. f and g A blockade of the NF-κB pathway increased γ-H2AX foci in irradiated NPC cells. h and i A blockade of the IL-8/NF-κB pathway increased the level of DNA damage in irradiated NPC cells. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001, ns, no significance