Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 1;40:85. doi: 10.1186/s13046-021-01884-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — Characteristics of 3 T-seq data. a, b Genomic locations of qualified 3 T-seq reads mapped to the reference genome. c, d Genomic distribution of the poly(A) sites. e, f The statistics of genes with various number of detected poly(A) sites. g, h Scatterplot of CULI for measuring the 3′UTR alteration in CPSF6-overexpressing or CPSF6 knockdown cells when compared with the corresponding control cells (false discovery rate (FDR) = 0.05). i CPSF6-modulated 7 candidate genes. These genes had the shortened 3′UTR in CPSF6-overexpressing HL-7702 cells and the lengthened 3′UTR in CPSF6 knockdown Huh-7 cells. j CPSF6-induced APA shift of NQO1. Left, Integrative Genomics Viewer (IGV) genome browser exhibited the poly(A) site usage of NQO1 3′UTR. Right, histogram showed the relative expression of the isoform with distal polyadenylation site (dPAS) relative to the one with proximal PAS (pPAS). k, l The protein levels of NQO1 in the CPSF6-overexpressing HL-7702 cells (k) and the CPSF6 knockdown Huh-7 cells (l) were assessed by western blotting. β-actin was used as internal control