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. 2021 Feb 3;118(8):e2018809118. doi: 10.1073/pnas.2018809118

Fig. 2.

Fig. 2.

Cell surface N terminomics with subtiligase-TM. (A) Workflow for cell surface N terminomics with subtiligase-TM. (B) Subcellular locations of N termini identified in a subtiligase-TM N terminomics experiment performed in HEK293T cells. (C) Topological locations of N-terminal peptides identified in the subtiligase-TM N terminomics experiment (Top) or a subtiligase lysate experiment (Bottom). (D) Distribution of membrane protein types observed in the subtiligase-TM N terminomics experiment. (E) Distribution of distances between cleavage sites identified in the subtiligase N terminomics experiments and the corresponding transmembrane domains.