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. 2021 Feb 15;118(8):e2019052118. doi: 10.1073/pnas.2019052118

Fig. 3.

Fig. 3.

ZMYND8 negatively regulates H3K27me3 target gene expression in ccRCC cells in culture and in patients. (A) 786-O and A498 cells transfected with control (sgControl) or two independent ZMYND8-specific sgRNAs (sgZMYND8-1 and sgZMYND8-2) were harvested for RT-qPCR analysis of expression of the indicated genes. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant. (B) WB analysis of the indicated proteins in lysate of 786-O and A498 cells transfected with sgControl or sgZMYND8. (C) Representative images of IHC with anti-ZMYND8 and anti-H3K27me3 antibodies on TMA (9 normal kidney and 41 ccRCC TMA elements) tissue sections. (D) Correlation analysis of IHC staining of ZMYND8 and H3K27me3 proteins in ccRCC patient specimens. (E) Meta-analysis of mRNA expression of indicated PRC2 target genes in ZMYND8 high and low TCGA patient samples. **P < 0.01; ***P < 0.001. (F) 786-O and A498 sgControl or sgZMYND8 cells were harvested for ChIP-qPCR analysis of the occupancy of indicated proteins at the RUNX2 gene locus. *P < 0.05; **P < 0.01; ***P < 0.001.