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. 2021 Feb 15;118(8):e2019052118. doi: 10.1073/pnas.2019052118

Fig. 6.

Fig. 6.

ZMYND8 is important for FOXM1 activation mediated by EZH2. (A) Heatmap showing expression of indicated MMP genes between sgControl and sgZMYND8 MDA-MB-231 cells under hypoxia. (B) sgControl or sgZMYND8 786-O and A498 cells were harvested for RT-qPCR analysis of mRNA expression of the indicated MMP genes. *P < 0.05; **P < 0.01. (C) Meta-analysis of indicated MMP genes at the mRNA level in ZMYND8 high and low TCGA ccRCC patient specimens. *P < 0.05; **P < 0.01. (D) sgControl or sgZMYND8 786-O and A498 cells were harvested for ChIP-qPCR analysis of the occupancy of indicated proteins at the MMP2 gene locus. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant. (E) WB analysis of co-IP of endogenous ZMYND8 and FOXM1 in 786-O cells. (F) WB analysis of co-IP of FOXM1 with Flag-tagged FL ZMYND8 or truncation mutants in 293T cells. (G) WB analysis of co-IP of endogenous EZH2 and FOXM1 in 786-O cells transfected with sgControl or sgZMYND8.