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. 2021 Feb 20;10(2):402. doi: 10.3390/plants10020402

Figure 2.

Figure 2

HPTLC chromatograms for the qualitative determination of flavan-3-ols and proanthocyanidins in STSs from rhizomes (1 μL, 50 mg/mL) of Japanese (track 6), Bohemian (track 7) and giant (track 8) knotweed based on standards. The HPTLC cellulose plates were developed with water (A), 1-propanol–water–acetic acid (4:2:1, v/v) (B), 1-propanol–water–acetic acid (20:80:1, v/v) (C), and documented at white light after derivatization with DMACA detection reagent. The ap plications of standards: (−)-gallocatechin gallate (60 ng; track 1), (−)-catechin gallate (60 ng; track 2), procyanidin C1 (150 ng; track 3), procyanidin B3 (100 ng; track 4), (−)-epicatechin (50 ng; track 5, higher RF), (−)-epigallocatechin (60 ng; track 5, lower RF), (+)-catechin (50 ng; track 9, higher RF), (−)-gallocatechin (60 ng; track 9, lower RF), (−)-epicatechin gallate (60 ng; track 10), procyanidin B1 (120 ng; track 11), procyanidin B2 (90 ng, track 12) (−)-epigallocatechin gallate (60 ng; track 13).