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. 2021 Feb 20;10(2):402. doi: 10.3390/plants10020402

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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HPTLC chromatograms used for the quantitative determination of proanthocyanidins in STSs from rhizomes (1 μL, 50 mg/mL) of Japanese (tracks 2 and 8), Bohemian (tracks 4 and 10) and giant (tracks 6 and 12) knotweed and standard solutions of (−)-epicatechin and procyanidin B2. The HPTLC silica gel plate was developed with toluene–acetone–formic acid (3:6:1, v/v) and documented at white light after derivatization with DMACA detection reagent. The applications of (−)-epicatechin and procyanidin B2 standard solutions: track 1: 30 ng; track 3: 40 ng; track 5: 60 ng; track 7: 80 ng; track 9: 100 ng; track 11: 120 ng; track 13: 150 ng.