Figure 6.

Studying the protective role of Yfh1 and Hfra on oxidation of α-syn. (A) MALDI-TOF/TOF signal of α-syn (10 μM) incubated during 0 min (black) and 150 min (blue and red) in the presence of AA (70 μM) (blue) or in the presence of AA (70 μM) and Cu²⁺ (2.5 μM) (red). (B) MALDI-TOF/TOF signals of Yfh1 (5 μM) and α-syn (10 μM) co-incubated with AA (70 μM) and Cu²⁺ (2.5 μM) during 0 min (black) and 150 min (red). (C) MALDI-TOF/TOF signals of Hfra (5 μM) and α-syn (10 μM) co-incubated with AA (70 μM) and Cu²⁺ (2.5 μM) during 0 min (black) and 150 min (red). (D) SDS-PAGE electrophoretic gel of the analysis of reaction mixtures containing 0.1mM EGS and: (1) 10 μM α-syn; (2) 10 μM Hfra; (3) 10 μM Yfh1; (4) 10 μM α-syn and 10 μM Hfra; and (5) 10 μM α-syn and 10 μM Yfh1. These mixtures were incubated during 30 min at 25 °C before analysis. The gel includes a marker (M) where each protein band has been labelled with its molecular weight (left).