Table 2.
The Central Composite Design (CCD) of experiments and response of Staphylococcus aureus phage pSa-3 production
| Standard order | Variables (coded value) | Variables (experimental value) | ||||
|---|---|---|---|---|---|---|
| Glycerol X1 |
Glycine X2 |
CaCl2 X3 |
Glycerol (mg) | Glycine (mg) | CaCl2 (mM) | |
| 1 | − 1 | − 1 | 1 | 250.0 | 25.0 | 15.0 |
| 2 | 1 | − 1 | − 1 | 750.0 | 25.0 | 5.0 |
| 3 | 1 | 1 | 1 | 750.0 | 75.0 | 15.0 |
| 4 | − 1 | 1 | − 1 | 250.0 | 75.0 | 5.0 |
| 5 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
| 6 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
| 7 | 1 | 1 | − 1 | 750.0 | 75.0 | 5.0 |
| 8 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
| 9 | − 1 | − 1 | − 1 | 250.0 | 25.0 | 5.0 |
| 10 | 1 | − 1 | 1 | 750.0 | 25.0 | 15.0 |
| 11 | − 1 | 1 | 1 | 250.0 | 75.0 | 15.0 |
| 12 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
| 13 | 1.633 | 0 | 0 | 908.0 | 50.0 | 10.0 |
| 14 | 0 | 0 | − 1.633 | 500.0 | 50.0 | 1.8 |
| 15 | 0 | 0 | 1.633 | 500.0 | 50.0 | 18.1 |
| 16 | 0 | − 1.633 | 0 | 500.0 | 9.0 | 10.0 |
| 17 | 0 | 1.633 | 0 | 500.0 | 90.0 | 10.0 |
| 18 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
| 19 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
| 20 | − 1.633 | 0 | 0 | 91.0 | 50.0 | 10.0 |