Table 3.

The experimental design and response of lytic Staphylococcus aureus phage pSa-3 production

Standard Order	Glycerol X1	Glycine X2	CaCl2 X3	Observed values ($\times$1010 PFU/mL)	Predicted values ($\times$1010 PFU/mL)
1	− 1	− 1	1	1.74 ± 1.42	4.81
2	1	− 1	− 1	4.54 ± 0.17	3.78
3	1	1	1	3.57 ± 0.29	3.78
4	− 1	1	− 1	8.31 ± 0.93	4.81
5	0	0	0	5.11 ± 0.41	4.36
6	0	0	0	5.12 ± 0.23	4.36
7	1	1	− 1	2.01 ± 0.21	3.77
8	0	0	0	5.09 ± 0.18	4.36
9	− 1	− 1	− 1	9.41 ± 2.08	4.81
10	1	− 1	1	2.88 ± 0.40	3.79
11	− 1	1	1	4.05 ± 1.85	4.81
12	0	0	0	5.06 ± 0.28	4.36
13	1.633	0	0	1.98 ± 0.42	3.35
14	0	0	− 1.633	5.26 ± 0.26	4.36
15	0	0	1.633	6.83 ± 0.69	4.36
16	0	− 1.633	0	7.07 ± 1.10	4.36
17	0	1.633	0	7.60 ± 1.24	4.35
18	0	0	0	5.20 ± 0.26	4.36
19	0	0	0	5.13 ± 0.31	4.36
20	− 1.633	0	0	4.05 ± 0.75	5.03