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. 2021 Feb 17;118(8):e2014941118. doi: 10.1073/pnas.2014941118

Fig. 1.

Fig. 1.

Generation and validation of a structural model predicting that the WDR41 7CD loop is necessary for interaction with PQLC2. (A) Ribbon diagram of the predicted WDR41 structure that forms an eight-blade β-propeller with a large loop (CD loop) formed between the C and D β-strands in blade 7. (B) Predicted structure of PQLC2 inward-facing conformation. PQ motifs in transmembrane helices 1 and 5 are highlighted. The position of PQLC2 in the lysosomal membrane was predicted via the PPM 2.0 server (30). (C) Model derived from the docking of predicted WDR41 and PQLC2 structures (model with the highest score shown) wherein the 7CD loop of WDR41 is inserted within the central cavity exposed by the inward-facing conformation of PQLC2. (D) WDR41 7CD loop amino acid sequence. (E) Anti-HA immunoprecipitation from cells expressing PQLC2-FLAG and HA-tagged wild-type or indicated mutant versions of WDR41-HA, followed by detection of the indicated proteins by immunoblotting. (F) Anti-HA immunoprecipitation followed by immunoblot detection of HeLa cells expressing PQLC2-FLAG along with either WT WDR41-HA or WDR41-HA mutants with the indicated deletions.