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. 2021 Feb 17;118(8):e2014941118. doi: 10.1073/pnas.2014941118

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Fig. 2. — A 10-aa peptide from the WDR41 7CD loop is sufficient for interaction with PQLC2. (A) Schematic representation of GFP chimeric proteins containing fragments of the WDR41 7CD loop. The indicated amino acid sequence of WDR41 regions of interest were fused to an unstructured C-terminal extension from GFP. (B) Analysis of PQLC2-FLAG binding to GFP-WDR41 chimeras by immunoprecipitation and immunoblotting. Proteins were immunoprecipitated with GFP-Trap beads, and samples corresponding to the lysates (Input) and immunoprecipitated proteins (IP: GFP) were immunoblotted with FLAG, GFP, and SMCR8 antibodies. (C) GST pull-down assay testing for an interaction between recombinant GST and GST-WDR41 TIP and PQLC2-FLAG from HeLa cell lysates. (D) PQLC2-FLAG was isolated by immunoprecipitation and used as bait to test for interaction with recombinant GST and GST-WDR41 TIP, respectively.