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. 2021 Mar 2;22:101. doi: 10.1186/s12859-021-04034-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Overview of the User Interface for PnB Designer. a Graphical layout of the user interface of the PnB Designer application in the ‘Multi Sample Run’ mode. In the first input fields, the user can select the editing strategy, either base- or prime editing and the running mode. When the ‘Multi Sample Run’ mode is selected, a CSV file with the user specified samples can be loaded into the application for batch analysis. b Template CSV file can be downloaded under the ‘Prime Editing template file’ link and has the format shown in panel b. c User interface in the ‘Single Sample Run’ mode. The editing location can be defined using genomic coordinates or as a raw text sequence